Concentrations (example)

Please note: All concentrations shown here are exemplatory. They do not reflect real data for the described products. Actual concentrations depend on the production lot of each product and can exclusively be found in the product information leaflet. Please ask for these product information leaflets or download them from the service site of the Chromsystems website.

Substance	Unit	Concentration Urine Calibration Standard	Concentration Aqueous Calibration Standard
Normetanephrine	µg/l nmol/l	736 4020	250 1365
Metanephrine	µg/l nmol/l	288 1459	250 1268
3-Methoxytyramine	µg/l nmol/l	300 1793	250 1495

Instructions

These Chromsystems calibrators are designed to monitor the accuracy and precision of analytical procedures for the quantitative determination of metanephrines in urine.

The lyophilised urine calibration standard (order no. 2009) is based on human urine and should be used routinely according to the instructions accompanying the assay procedure being used. While following these instructions, the urine calibration standard is to be prepared and injected in the same manner as a patient specimen. .

The aqueous calibration standard (order no. 2023) is injected directly into the HPLC system without sample preparation.

Reconstitution

(Urine Calibration Standard)

Remove the metal seal and rubber stopper from the vial. Add exactly 5.0 ml of double distilled water (possibly HPLC-grade) into the vial. Replace the stopper on the vial and allow to sit for 10 to 15 minutes at room temperature. Swirl the vial to dissolve the contents until homogeneity.

Storage conditions

(Urine Calibration Standard)

Stored at <-18 °C unopened calibration standards are stable until the expiry date which is stated on the vial labels. The reconstituted calibration standard should be stored tightly capped at +2 to +8 °C. The prepared calibration standard sample has the same stability as a fresh urine specimen. Use the known storage conditions for the different components. For specific information about the stability of the individual components please refer to the instructions of your test instruction manual. Generally the calibration standard can be stored for two days at +2 to +8 °C. If the calibration standard cannot be analysed within this period, it should be aliquoted and stored frozen at <-18 °C.

Ordering information

2009	Metanephrines Urine Calibration Standard (lyoph.)	5 x 5 ml
2023	Calibration Standard for analysis of metanephrines in urine (primary standard solution)	10 ml

Additionally available:

0040	Endocrine Urine Control, normal range	10 x 8 ml
0050	Endocrine Urine Control, pathological range	10 x 8 ml