Concentrations (example)
Please note: All concentrations shown here are exemplatory. They do not reflect real data for the described products. Actual concentrations depend on the production lot of each product and can exclusively be found in the product information leaflet. Please ask for these product information leaflets or download them from the service site of the Chromsystems website.
| Substance | Unit | Value
Level I | Range
Level I | Value
Level II | Range
Level II | Value
Level III | Range
Level III |
| LC-MS/MS |
| 25-OH-Vitamin D3 | µg/l
nmol/l | 16.7
41.7 | 13.4-20.1
33.4-50.1 | 38.4
95.8 | 30.7-46.1
76.6-115 | 104
260 | 83.3-125
208-312 |
| 25-OH-Vitamin D2 | µg/l
nmol/l | 17.4
42.1 | 13.9-20.9
33.7-50.5 | 38.7
93.9 | 31.0-46.5
75.1-113 | 94.6
229 | 75.7-114
183-275 |
| HPLC |
| 25-OH-Vitamin D3 | µg/l
nmol/l | 29.0
72.4 | 23.2-34.8
57.9-86.9 | 88.9
222 | 71.1-107
178-266 | ---- | ---- |
| 25-OH-Vitamin D2 | µg/l
nmol/l | 27.7
67.1 | 22.2-33.2
53.7-80.6 | 84.3
204 | 67.4-101
163-245 | ---- | ---- |
Instructions
These Chromsystems serum controls are designed to monitor the accuracy and precision of analytical procedures for the quantitative determination of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum/plasma. The lyophilised control is based on human serum and should be used routinely according to the instructions accompanying the assay procedure being used. While following these instructions the control is to be handled in the same manner as a patient specimen
Reconstitution
Remove the metal seal and rubber stopper from the vial. Add exactly 1.0 or 2.0 ml of double distilled water (possibly HPLC-grade) into the vial. Replace the stopper and allow to sit for 10 to 15 minutes at room temperature. Swirl the vial to dissolve the contents until homogeneity.
Storage conditions
Stored at ≤-18 °C unopened controls are stable until the expiry date which is stated on the vial labels. The reconstituted control can be stored up to one week light protected and tightly capped at +2 to +8 °C. If the controls cannot be analysed within this period, they should be aliquoted and stored frozen at ≤-18 °C (up to 3 months).
Ordering information
| LC-MS/MS |
| 0221 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Bi-Level | 2 x 5 x 1 ml |
| 0222 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level I | 5 x 1 ml |
| 0223 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level II | 5 x 1 ml |
| 0256 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level III | 5 x 1 ml |
| HPLC |
| 0028 | 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Bi-Level | 2 x 5 x 2 ml |
| 0029 | 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level I | 5 x 2 ml |
| 0030 | 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level II | 5 x 2 ml |
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